Orochimaru is one of the three legendary Sannins. He is someone who wants to learn all the techniques there exist in this world. Since he cannot achieve this in one life, he changes bodies so he can stay young. Since he is capable of using alot of techniques medical ninjutsu is one of them. He has mastered medical ninjutsu and because of which he can keeo on healing himself and extend his life more and more. Also we have seen him using medical ninjutsu when he was a Konohagakure’s shinobi.
3. Tsunade Senju
Tsunade Senju is one of the Legendary Sannin of Konohagakure. She excells in medical ninjutsu. She has excellent hold over her chakra. She has saved countless no. of lives during several wars. She also played a crucial role when Konohagakure was attacked by Pein. She can store her chakra to use strength of the hundred seals. She can also transfer her chakra to others.